Premium
DETECTION OF MOUSE MACROPHAGE ANTIGENS BY ANTIMACROPHAGE SERUM
Author(s) -
Tiller Glenis E,
MeGiven AR,
Phil E
Publication year - 1973
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1973.75
Subject(s) - macrophage , antigen , antiserum , peritoneal cavity , spleen , bone marrow , biology , lung , microbiology and biotechnology , immunology , pathology , chemistry , medicine , in vitro , anatomy , biochemistry
Summary An antimacrophage serum prepared in a rabbit injected with BALB/c mouse peritoneal macrophages detected a macrophage‐specific antigen on peritoneal macrophages and a second macrophage antigen shared with polymorphonuclear leucocytes and fibroblasts. The macrophage‐specific antigen was present in the four mouse strains examined, in one of three strains of rats and in guinea‐pigs The specific peritoneal macrophage antigen and the antigen shared with polymorphs were detectable only after culture of macrophages from bone marrow, spleen, Lung and pleural cavity, isolated Kupffer cells, whether cultured or otherwise, showed no reaction with the antisera. Ultrastructurally, the specific macrophage antigen was localized to the cell membrane as discrete electron‐dense deposits of 25‐40 nm, regularly distributed 30‐50 nm apart.