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PATHOGENESIS OF THE GRAFT‐VERSUS‐HOST REAGTION IN CHICKEN EMBRYOS. REQUIREMENT OF YOLK SAC‐DERIVED STEM CELLS FOR THE DEVELOPMENT OF PROLIFERATIVE LESIONS
Author(s) -
Walker Karen Z,
Laffehty KJ,
Schoefl Gutta I
Publication year - 1973
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1973.33
Subject(s) - yolk sac , embryo , biology , andrology , stem cell , chorioallantoic membrane , haematopoiesis , yolk , embryonic stem cell , transplantation , pathogenesis , immunology , anatomy , microbiology and biotechnology , medicine , genetics , ecology , gene
Summary Randomly bred chicken enibryos were inoculated on days 5, 6 and 10 with adult AA blood and examined on day 13. Widespread haemorrhages occurred on the body surface, the yolk sac was poorly developed and haemopoietic tissue of the yolk sac folds was severely depleted. Lymphopoiesis in the thymus was depressed and the differentiation of the bursa of Fabricius was retarded. These lesions are characteristic of a graft‐versus‐host reaction in very young (rather than 10‐day‐old) embryos and appear to result from the destruction of haemopoietic stem cells in the yolk sac thus preventing their dissemination to intra‐embryonic haemopoietic organs. Since it has been suggested that yolk sac‐derived stem cells are required for the development of proliferative lesions during a graft‐versus‐host reaction in older embryos, the pock‐forming ability of these embryos depleted of stem cells by an early graft‐versus‐host reaction was examined. Randomly bred embryos inoculated on days 5 and 6 developed very few pocks in response to a third inoculation of adult AA blood on day 10. In comparison, AA chicken embryos inoculated on days 5 and 6 with adult AA blood before inoculation of adult allogeneic blood onto the chorioallantoic membrane on day 10 developed normal numbers of pocks. These results support the hypothesis that yolk sac‐derived stem cells are required for the development of proliferative lesions during a graft‐versus‐host reaction.