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THE UTILIZATION OF ZWITTERIONIC BUFFER SYSTEM IN THE PLAQUE ASSAY OF A FELINE CALICIVIRUS
Author(s) -
Love Daria N
Publication year - 1973
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1973.25
Subject(s) - feline calicivirus , infectivity , titration , phosphate buffered saline , virology , calicivirus , chemistry , virus quantification , tris , virus , biology , chromatography , biochemistry , inorganic chemistry
In 1971 feline picornaviruses were placed with vesicular exanthema virus in the genus Calicivirus within the family Picornaviridae (Wildy, 1971). Several publications utilizing the tube culture technique for infectivity titrations of feline caliciviruses have appeared (Burki, 1965; Burki and Pichler, 1971). Kahn and Gillespie (1970) mentioned plaque formation by strains of feline caliciviruses but no plaque assay technique has previously been described. Although Good, Winget, Winter, Connolly, Izawa and Singh (1966) first reported the development and the possible application of certain zwitterionic amino acids as buffering systems for biological research, most virus plaque assay techniques still utilize either closed or open systems with carbon dioxide supplementation. However, Richter (1967) reported the use of a zwitterionic buffer N‐tris (hydroxymethyl) methyl‐2‐aminoethane sulphonic acid (TES) in an open system which gave results comparable to bicarbonate‐carbon dioxide buffering in air. This communication reports on the use of a zwitterionic amino acid buffer system in the development of a plaque technique for feline caliciviruses.