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OUABAIN‐INDUCED GROWTH RETARDATION AND DEATH IN CHICK EMBRYOS
Author(s) -
Edwards Anne,
Gupta JD,
Harley JD
Publication year - 1972
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1972.71
Subject(s) - ouabain , embryo , incubation , saline , growth retardation , medicine , endocrinology , biology , triphosphatase , andrology , adenosine triphosphatase , enzyme , chemistry , biochemistry , atpase , pregnancy , sodium , microbiology and biotechnology , genetics , organic chemistry
Summary Ouabain injected into the air space of fertile hen eggs on the seventh day of incubation produced retardation of growth of the embryos incubated over a further period of five days. An injected close of 35 μg of ouabain per embryo caused 24% mortality of the eggs as against 9% in the saline‐injected controls. The survivors showed marked retardation of growth as ouabain‐treated embryos had a significantly lower weight than the saline‐injected controls. On doubling the dose of ouabain, the mortality rate increased to 57% and the growth retardation in the survivors was even more pronounced. Apart from growth inhibition, no gross structural abnormalities were noticed. The activity of the enzyme Na + ‐K + activated adenosine triphosphatase was depressed in the erythrocytes of the ouabain‐treated chicks.