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THE SEPARATION OF DIFFERENT CELL CLASSES FROM LYMPHOID ORGANS
Author(s) -
Shortman Ken,
Byrd W,
Williams N,
Brunner KT,
Cerottini JC
Publication year - 1972
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1972.26
Subject(s) - phytohaemagglutinin , cytotoxic t cell , spleen , pokeweed mitogen , biology , cell , microbiology and biotechnology , b cell , lymphocyte , chemistry , antibody , immunology , concanavalin a , biochemistry , in vitro
Summary Passage of lymphoid cell suspensions through glass‐bead columns separated lymphotytes into “adherent” and “non‐adherent” populations on the basis of a temperature‐independent. “physical adherence” effect. Density distribution analysis on continuous albumin gradients demonstrated that adherent cells were gererally light, and non‐adlierent cells were generally dense. The “B‐cell” and “T‐cell” classes of lymphocyte in mouse spleen both contained light adherent and dense non‐adherent components. The adherence columns and the density procedure both separated sub‐populations within the “B‐ceir” and the “T‐cell” lineages. Lymphocytes in mouse spleen responsive to phytohaemagglutinin mitogen were dense non‐adherent cells, whereas those responsive to pokeweed mitogen were predominantly light adherent cells. Antibody‐forming cells (“B‐cell” lineage) and cytotoxic lymphocytes (“T‐cell” lineage) were predominantly, but not exclusively, light adherent cells.