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INTERACTION OF EMBRYONIC CHICKEN SPLEEN CELLS AND ADULT ALLOGENEIC LEUCOCYTES
Author(s) -
Killby Virginia AA,
Lafferty KJ,
Ryan Madeleine A
Publication year - 1972
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1972.25
Subject(s) - chorioallantoic membrane , spleen , embryonic stem cell , biology , embryo , mitomycin c , stimulation , microbiology and biotechnology , cell , immunology , genetics , endocrinology , gene
Summary Leucocytes from inbred AA chickens produced pocks when inoculated onto the chorioallantoic membrane of non‐inbred embryos ( direct pock formation), but produced very few or no pocks on the chorioallantoic membrane of syngeneic embryos. Pock formation was observed when AA leucocytes were mixed with non‐inbred embryonic spleen cells before being assayed on the chorioallantoic membrane of recipient embryos that were unresponsive to either cell population alone ( indirect pock formation). Indirect pock formation was found to be more radio‐resistant than direct pock formation and doses of Mitomycin‐C that inhibited direct pock formation by AA leucocytes did not inhibit the capacity of these cells to induce indirect pocks. Indirect pock formation was inhibited when non‐inbred embryonic spleen cells were treated with Mitomycin‐C prior to mixing with AA leucocytes. It is suggested that indirect pock formation does not result from the stimulation of adult leucocytes by antigen presented on the surface of allogeneic embryonic cells. Rather, this response is a manifestation of the direct stimulation of embryonic spleen cells by adult allogeneic leucocytes.