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IN VITRO EFFECT OF RUBELLA VIRUS ON EMBRYONIC CHICK LENS
Author(s) -
Harley JD,
Edwards Anne,
Filipic M,
Gupta JD
Publication year - 1971
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1971.62
Subject(s) - rubella virus , virus , incubation , rubella , biology , virology , incubation period , lens (geology) , embryonic stem cell , tissue culture , in vitro , microbiology and biotechnology , andrology , medicine , vaccination , genetics , biochemistry , measles , paleontology , gene
Summary Lenses dissected from 12‐day embryonic chicks, then incubated with rubella virus for varying periods of time, were subsequently found to have a shorter metabolic life in organ culture than those treated with uninfected RK13 cell extracts. In high concentrations of virus this inhibitory effect was rapid; with lower concentrations or shorter treatment time the effect was delayed. Virus could be isolated from the supernatant culture media 48 hours after removal of the original virus inoculum, but not subsequently. Distinct histopathological changes were usually apparent in lenses after 4 hours' incubation with rubella virus suspension, and extensive changes were present after 24 hours' incubation. Extract's of rubella‐infected RK13 cells from which rubella virus had been eliminated were also toxic to the lens. The early changes in this experimental system thus appear due to a direct toxic effect; the later changes may result from viral proliferation in the lens.