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INHIBITION OF BONE MARROW COLONY FORMATION IN VITRO BY DIALYSABLE PRODUCTS OF NORMAL AND NEOPLASTIC HAEMOPOIETIC CELLS
Author(s) -
Metcalf D
Publication year - 1971
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1971.37
Subject(s) - bone marrow , in vitro , spleen , biology , lymph node , haematopoiesis , immunology , precursor cell , cancer research , chemistry , microbiology and biotechnology , biochemistry , stem cell
Summary Dialysates of tissue culture medium conditioned by normal cells (bone marrow, thymic, spleen or lymph node) or by leukaemic cells (myelomonocytic, lymphoid or plasma cell tumour) inhibited colony formation in agar by mouse bone marrow cells. The dialysable inhibitors were not species specific, did not alter colony morphology and were heat‐ and ether‐resistant. Leukaemic cells were as effective in producing inhibitors as normal cells, and colony formation in vitro by leukaemic cells was as susceptible to inhibition as was colony formation by normal cells. No dear evidence was obtained for the presence of similar dialysable inhibitors in the serum of normal or experimental mice, and the real biological function of these dialysable inhibitors remains in doubt.