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ENZYME TREATMENT OF COLONY STIMULATING FACTOR: EVIDENCE FOR A PEPTIDE COMPONENT
Author(s) -
Stanley ER,
Metcalf D
Publication year - 1971
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1971.28
Subject(s) - proteolysis , enzyme , peptide , subtilisin , chymotrypsin , colony stimulating factor , biology , trypsin , biochemistry , proteolytic enzymes , chemistry , microbiology and biotechnology , haematopoiesis , stem cell
Summary The effects of various proteolytic enzymes, glycosidases and phos‐ pholipases on the bone marrow colony stimulating factor from human urine have been investigated. Colony stimulating factor Was found to be comparatively resistant to inactivation by all of the enzymes tested with the exception of α‐chymotrypsin (at low concentrations), subtilisin and ficin. When biological activity remained following enzyme treatment, no change could be detected in the behaviour of colony stimulating factor on polyacrylmide disc electro‐ phoresis, under conditions in which a sieving effect on colony stimulating factor migration was observed. The studies have indicated the existence of a peptide component of colony stimulating factor, its relative resistance to enzymatic proteolysis and its presence as a minor fraction of the total protein in the urine of normal humans.