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A MODIFIED ANTIBODY FORMING CELL ASSAY FOR DETECTING CELLS PRODUCING A PARTICULAR CLASS OF ANTIBODY
Author(s) -
Kraft N,
Wistar R
Publication year - 1971
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1971.2
Subject(s) - antibody , flagellin , immune system , microbiology and biotechnology , immunoglobulin m , chemistry , biology , immunology , immunoglobulin g , receptor , biochemistry
Summary A modified immune cytoadherence assay is described which is capable of distinguishing cells forming IgM antibody from other types of antibody producing cells in the rat. The kinetics of appearance of IgM and non‐IgM producing antibody forming cells in fixed lymphoid tissues and in blood were determined using this modified assay. Non‐IgM forming cells were found early in the primary response to Salmonella adelaide polymerized flagellin protein, and no evidence was seen to suggest a sudden switch from IgM to IgG production.