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DISTRIBUTION VOLUMES OF LABELLED RED BLOOD CELLS AND LABELLED PROTEIN AND “F CELLS” IN INTACT AND SPLENECTOMIZED HbA AND HbB TYPE SHEEP
Author(s) -
Dooley PC,
Morris RJH,
Harris LRJ
Publication year - 1971
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1971.12
Subject(s) - splenectomy , blood volume , volume of distribution , red blood cell , red cell , volume (thermodynamics) , chemistry , plasma volume , endocrinology , medicine , spleen , pharmacokinetics , thermodynamics , physics
Summary The dilutions of labelled red blood cells and labelled albumin were studied over a 10 hour period in HbA and HbB sheep, both before and after splenectomy. The dilution curves were analysed into exponentials, and values for red blood cell volume and plasma volume were calculated from various portions of the curves. Dilution curves for labelled red blood cells in intact sheep showed. important differences from those in splenectomized sheep. Red blood cell volume was calculated from: The extrapolation of the exponential process with the slowest decay rate; the sum of intercepts of all exponential processes; jugular haematocrit and plasma volume. Splenectomy significantly reduced the values for these parameters. Plasma volume was calculated from the extrapolation of the exponential with the slowest decay rate and from the sum of intercepts of all exponential processes. Splenectomy significantly reduced the values for these parameters. Blood volume and the value for the ratio of body to jugular haematocrit were calculated from the data, and were found to be significantly reduced by splenectomy. Differences between HbA and HbB sheep were found only for jugular haematocrit and red blood cell volume calculated from jugular haematocrit and plasma volume. The differences in values from the several methods of calculating red blood cell volume in intact sheep and the reduction of these values by splenectomy are discussed in relation to the splenic red blood cell content.