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ADHERENCE‐PROMOTING ANTIBODIES. THE INVESTIGATION OF PHYSICOCHEMICAL PROPERTIES OF MOUSE ANTI‐VI ANTIBODIES PROVIDING IMMUNE FIXATION OF ANTIGEN TO MACROPHAGE SURFACE.
Author(s) -
Levenson VI,
Braude Nelli I,
Chernokhvostova Elena V
Publication year - 1970
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1970.44
Subject(s) - antibody , antigen , chemistry , phagocytosis , macrophage , in vitro , immunology , immunization , immune system , ultracentrifuge , biochemistry , medicine
Summary Anti‐Vi antibodies present in the serum of mice shortly after immunization have been shown to provide adherence of antigen to the surface of macrophages. In the present study the physicochemical properties of these antibodies were investigated. DEAE‐Cellulose column chromatography and sucrose gradient ultracentrifugation have revealed that all this activity is confined to the IgM fraction of serum. These findings are consistent with the observation that treatment of early anti‐Vi sera with cysteine or 2‐mercuptoethanol completely destroys their agglutinating and adherence‐promoting activities. In addition, it has been found that antibodies which promote in vitro phagocytosis of antigen by macrophages belong to the IgG class of immunoglobulins. The possible implications of these findings are discussed in detail.