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THE TRANSFER OF IMMUNOLOGICAL COMPETENCE TO RATS TOLERANT OF SHEEP ERYTHROCYTES WITH LYMPHOCYTES FROM NORMAL RATS
Author(s) -
McCullagh PJ
Publication year - 1970
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1970.38
Subject(s) - thoracic duct , immune system , antigen , immunology , incubation , biology , lymphocyte , in vitro , hemolysin , adoptive cell transfer , immunity , lymphatic system , t cell , biochemistry , virulence , gene
Summary An explanation has been sought for the mechanism whereby prior irradiation of rats tolerant of sheep erythrocytes facilitates haemolysin formation by transfused normal lymphocytes. Taking account of the effects of depleting tolerant rats of lymphocytes by means of prolonged thoracic duct drainage, it was concluded that enhanced colonization of host lymphoid tissues by transfused cells was unlikely to be the major factor in this facilitation. The formation of haemolysin was investigated in tolerant rats after sub lethal irradiation and the transfer of lymphocytes from normal donors. The haemolysin response after the transfer of thoracic duct lymphocytes to irradiated, tolerant hosts was much weaker than that observed after their transfer to irradiated normal (non‐tolerant) hosts unless the lymphocytes had been incubated in vitro with antigen primed peritoneal cells. Previous incubation of the lymphocytes to be injected with unprimed peritoneal cells or with antigen alone failed to enhance the subsequent immune response. Lymphocytes and primed peritoneal cells which had not been incubated together were a much less efficient inoculums than incubated cell mixtures. It is suggested that, in addition to the specific deficiency of its lymphocytes, there is some factor in the tolerant rat inimical to the initiation of an immune response in transfused normal lymphocytes.