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HUMORAL AND CELLULAR RESPONSES OF MICE TO A PNEUMOCOCCAL POLYSACCHARIDE ANTIGEN.: PLAQUES AND PARALYSIS, IN VIVO AND IN VITRO
Author(s) -
Kearney R,
Halliday WJ
Publication year - 1970
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1970.22
Subject(s) - antibody , antigen , immune system , in vitro , spleen , in vivo , biology , immunology , microbiology and biotechnology , biochemistry
Summary Cells forming the haemolytic type of anti‐SIII antibody were counted in muse spleens by a plaque method. Plaque‐forming cells were shown to be present after low doses of antigen (when haemagglutinating and haemolytic antibodies were circulating) and after moderately high doses (when only haemolytic antibodies were detectable), but not after very high doses (when all circulating antibody was absent). Plaque‐forming cells were also found under certain conditions (early in the immune response or in the presence of high titres of haemagglutinating antibody) when haemolytic antibody was not apparent in the serum. These findings are discussed in terms of competition between the two antibodies and preferential neutralization of haemagglutinating antibody by antigen. As far as haemolytic antibody is concerned, immunological paralysis after high doses of SIII appears to be a central inhibition of antibody‐forming cells. Primary immune responses in vitro were demonstrated with normal mouse spleen cells treated with SIII. A moderate dose of antigen elicited significant numbers of specific plaque‐forming cells. A high dose produced a situation analogous to paralysis.