Premium
ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS
Author(s) -
Percival Lorraine H,
Dunckley GG,
Purves HD
Publication year - 1970
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1970.16
Subject(s) - polyacrylamide , isoelectric focusing , polyacrylamide gel electrophoresis , acetic acid , chromatography , chemistry , biochemistry , polymer chemistry , enzyme
Summary An improved technique for isoelcctric focusing of proteins in polyacrylamide gels is described. In studying insulin with the procedure, mercuric chloride in trichloro‐acetic acid was used to fix the protein in the gel. One major and five minor components were demonstrated in ox and pig insulin.