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THE BEHAVIOUR OF TWO STRAINS OF MURINE SARCOMA VIRUS IN VITRO
Author(s) -
Simons PJ
Publication year - 1970
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1970.10
Subject(s) - subculture (biology) , virus , biology , multinucleate , sarcoma , virology , in vitro , staining , giant cell , cell culture , microbiology and biotechnology , pathology , genetics , medicine
Summary Two variants of murine sarcoma virus were able to cause different alterations in an established line of mouse embryo cells. Infection with the Moloney strain of murine sarcoma virus produced densely staining round cells and spindle cells which were overgrown by normal cells on passage. The Harvey strain of murine sarcoma virus induced the formation of densely staining round cells and spindle cells which multiplied on subculture. Passage of murine sarcoma virus (Harvey) infected cultures led to the appearance of multinucleate giant cells. Murine sarcoma virus (Harvey) transformed cells were able to divide and form colonies in soft agar. Lines of murine sarcoma virus (Harvey) transformed cells have been established from soft agar colonies.