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ELECTROPHORESIS OF HUMAN SALIVA AND IDENTIFICATION OF INHERITED VARIANTS OF AMYLASE ISOZYMES
Author(s) -
Boettcher B,
Lande Frances A
Publication year - 1969
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1969.9
Subject(s) - isozyme , saliva , locus (genetics) , staining , amylase , starch , biology , polyacrylamide gel electrophoresis , microbiology and biotechnology , biochemistry , gene , genetics , enzyme , chemistry
Summary Saliva specimens have been examined electrophoretically, using polyacrylamide gel slabs. By slicing the stabs in halves, incubating one half‐gel in starch followed by Staining with iodine and staining the other half‐gel with amido black, amylase isozymes are visualized as bands of enzymic activity, up to 8 in number, 4 of which can usually be seen on amido black staining. Several different isozyme patterns have been observed. The results of pedigree studies suggest simple modes of inheritance of each of the variants. However, because different variants each involve only one or two of the isozymes, it appears that more than one structural gene locus is involved in the production of salivary amylase isozymes.