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DISTRIBUTION OF 125 I‐IMMUNOGLOBULINS, IgG SUBUNITS AND ANTIGEN‐ANTIBODY COMPLEXES IN RAT LYMPH NODES
Author(s) -
Herd Zana L,
Ada GL
Publication year - 1969
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1969.6
Subject(s) - antibody , lymph , microbiology and biotechnology , heterologous , chemistry , bovine serum albumin , rabbit (cipher) , antiserum , human serum albumin , albumin , serum albumin , immunoglobulin g , antigen , biology , biochemistry , immunology , pathology , medicine , gene , mathematics , statistics
Summary. The ability of rabbit IgG to localize in follicles of rat lymph nodes (heterologous system) has been shown to be due to the Fc fragment. Of the rabbit IgG subunits examined, the H chain and Fc fragment showed follicular localization in rats, while the L chain and Fab fragment did not. Two large peptides from the Fc region of the molecule, C3 and C4, also showed follicuiar localization. Human serum albumin showed follicular localization in rats only when it was complexed with rabbit anti‐human serum albumin intact IgG. Complexes of human serum albumin with rabbit anti‐human serum albumin Fab fragment did not go to the follicles. When major subunits of rabbit IgG were injected into rabbits (homologous system) results were qualitatively the same as those obtained in rats, with one exception. Rabbit Fab localized in follicles of rabbit nodes. It was also found the rat Fab localized in rat follicles. Purified rat IgM has been shown to localize well in rat follicles. All substances examined have shown medullary localization.