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THE DEVELOPMENT OE CLONES OE ANTIBODY‐FORMING CELLS IN THE SPLEENS OE IRRADIATED MICE
Author(s) -
Cunningham AJ
Publication year - 1969
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1969.52
Subject(s) - spleen , antibody , microbiology and biotechnology , biology , irradiation , chemistry , pathology , immunology , medicine , physics , nuclear physics
Summary A markedly non‐random distribution of antibody‐plaque‐forming cells was found in the spleens of lethally irradiated mice which had been injected 5–10 days previously with sheep erythrocytes and 1−2 × 10 6 syngeneic spleen cells. Localised concentrations (colonies) of 19S and 7S plaque‐forming cells were identified by cutting the spleens transversely into 8 pieces, making dispersed‐cell suspensions from these segments, and testing the suspensions directly with the haemolytic plaque technique. The colonies had an average length of approximately 0.5 mm., with an upper size limit of about 1.5 mm. There was little correlation between the position of these colonies and the position of local concentrations of haemolytic antibody detected by a haemolytic focus method.