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“PREGNANCY PROTEIN” IN POLYACRYLAMIDE GRADIENT‐GEL ELECTROPHORESIS
Author(s) -
Margolis J,
Kenrick KG
Publication year - 1969
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1969.158
Subject(s) - pregnancy , haptoglobin , electrophoresis , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , chemistry , medicine , chromatography , endocrinology , biology , biochemistry , genetics , enzyme
Summary A protein band appearing in gradient gel electrophoresis between the second and third terms of the haptoglobin 2–1 haemoglobin complex has been demonstrated in the sera of pregnant women and of women taking oestrogen‐containing oral contraceptive pills. The appearance in the serum of this “pregnancy protein” has been shown to be oestrogen‐dependent. The location of the pregnancy protein just below the Hp.Hb (2–1) trimer in the gradient gel implies a molecular weight of around 450,000. The gradient technique gives sharper resolution of the pregnancy protein and appears to be more sensitive than the electrophoretic methods previously used.