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THE SIZE AND DENSITY DISTRIBUTION OF FOWL BLOOD LYMPHOCYTES INITIATING A GRAFT‐VERSUS‐HOST REACTION
Author(s) -
Shortman Ken,
Szenberg A
Publication year - 1969
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1969.1
Subject(s) - buffy coat , differential centrifugation , centrifugation , fowl , population , bead , lymphocyte , percoll , chemistry , biology , chromatography , immunology , biophysics , microbiology and biotechnology , materials science , paleontology , demography , sociology , composite material
Summary. The cells in fowl blood capable of initiating a graft‐versus‐host reaction have been characterised using a series of separation methods. Lymphocytes were separated from other cells by preparation of a buffy‐coat fraction followed by passage through cotton‐wool columns to remove adherent cells. The lymphocytes were then separated by size using filtration through cold, small, glass bead columns, or by density using centrifugation in albumin gradients. Purified lymphocytes were fully active. The majority of the active cells behaved like small lymphocytes on the glass head columns, However, activity appeared to be carried by a small sub‐population of cells, distinguishable by their density distribution from the bulk of circulating lymphocytes, and distinct from the total population of small lymphocytes. The graft‐versus‐host activity showed marked density heterogeneity, with well defined peaks. This suggests that active cells exist in many distinct metabolic or differentiation states or, alternatively, that different lines of cells are active. The density distribution of activity and of lymphocytes varied from one bird to another. The basis for the variation is discussed.