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SOME FACTORS INFLUENCING THE REACTIONS OF HUMAN SYNOVIAL CELLS IN VITRO WITH FRESH HOMOLOGOUS AND AUTOLOGOUS SERUM
Author(s) -
Clarris BJ,
Fraser JRE
Publication year - 1968
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1968.9
Subject(s) - hyaluronidase , trypsin , in vitro , homologous chromosome , stimulation , andrology , chemistry , inoculation , serum concentration , medicine , endocrinology , immunology , biology , microbiology and biotechnology , biochemistry , enzyme , gene
Summary Multiplication of human synovial cell strains was studied in media containing homologous or autologus serum. When serum was applied to cell cultures 24 hr. after attachment and spreading, the effects of fresh serum relative to heat‐inactivated serum were as follows: (a) In 10% concentration, growth was often stimulated by fresh serum; (b) in 50% concentration, some individual specimens of fresh serum caused stimulation or slight depression of growth, but a series of observations showed no consistent difference in the effects of fresh and heat‐inactivated serum at this level; (c) in 100% concentration, fresh serum caused a consistent and significant depression of growth; (d) autologous fresh serum caused a similar depression of growth. Treatment of cultures with bacterial hyaluronidase caused a slight but definite enhancement of the inhibitory effect of fresh serum. When serum was applied to cells in suspension immediately after treatment with trypsin, the inhibitory effect of fresh serum was much greater and morphological changes were apparent. The implications of these findings are discussed.