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A COMPARATIVE ASSAY OF O‐SOMATIC ANTIGEN 5 OF SALMONELLAE
Author(s) -
Auzins Ieva
Publication year - 1968
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1968.8
Subject(s) - hemagglutination , polysaccharide , antigen , microbiology and biotechnology , bacteria , strain (injury) , virulence , chemistry , galactose , antibody , biology , biochemistry , immunology , gene , genetics , anatomy
Summary A method is described for determining the amount of antigen 5 (O‐acetyl galactose) present in a number of strains of Salmonellae. Using haemagglutination inhibition reactions specific for antigen 5, a measure was made of the ability of various strains of bacteria and the lipopolysaccharides and polysaccharides extracted from these bacteria to produce haemagglutination inhibition. The O‐acetyl contents of the various polysaccharides were determined using the Hestrin technique. Because good correlation was found between the O‐acetyl content of the polysaccharides and their ability to produce haemagglutination inhibition, estimations of the amount of antigen 5 available for reaction with antibody on the surface of a virulent (C5) and an avirulent (M206) strain of S. typhimurium were made, based on the assumption that each O‐acetyl group detected in the polysaccharide represents one antigen 5 site. It was foimd that the M206 strain contains half the number of antigen 5 reactive groups that are present on the surface of the C5 strain.