Premium
HISTOCOMPATIBILITY ANTIGENS IN THE RAT: THE AS2 STRAIN IN RELATION TO THE AS, BS AND HS STRAINS
Author(s) -
Heslop Barbara F
Publication year - 1968
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1968.41
Subject(s) - locus (genetics) , histocompatibility , biology , antigen , allele , spleen , genetics , major histocompatibility complex , strain (injury) , gene , microbiology and biotechnology , immunology , human leukocyte antigen , anatomy
Summary The origin of the inbred albino rat strain AS2 is described. With the exception of weak male → female incompatibility, the strain appears to be isohistogenic and homozygous at all loci determining histocompatibility antigens. The weak antigen distinguishing males from otherwise isohistogenic females is identical in the AS2 and HS strains. The AS2 strain possesses at least one histocompatibility antigen in common with each of strains AS, BS and HS. The AS. BS and HS strains possess identical alleles at a major histocompatibility locus at which they differ from the AS2 strain. Donor‐host disparity at this locus regularly leads to the development of local graft versus host reactions following the injection of 50 × 10 6 normal parental strain spleen cells beneath the renal capsule of F1 hybrids. Where donor and host are compatible at this locus, despite disparity at up to 3 other loci determining strong antigens, local graft versus host reactions do not occur following normal spleen cell transfer, although they occur somewhat irregularly if specifically sensitised spleen donors are used. It therefore seems likely that this major locus is the Ag‐B locus. Mean skin graft survival times are only slightly shorter in Ag‐B incompatible combinations than in those combinations which are compatible at Ag‐B hut differ in respect of other strong histocompatibility antigens. Parental strain → F2 hybrid skin grafts show the closely related AS and AS2 strains to differ from each other at an estimated 4‐5 histocompatibility loci, one of which is the Ag‐B locus.