Premium
COLONY GROWTH OF RAT BONE MARROW CELLS IN VITRO
Author(s) -
Bradley TR,
Siemienowicz R
Publication year - 1968
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1968.166
Subject(s) - bone marrow , in vitro , peripheral blood mononuclear cell , incubation , plating efficiency , biology , agar , microbiology and biotechnology , pathology , chemistry , immunology , medicine , biochemistry , genetics , bacteria
Summary A method is described which is suitable for the growth of colonies from rat bone marrow cells in vitro . The method involves plating out rat marrow cell suspensions in agar, using small amounts of rat serum as the stimulus to colony growth. Dose response relationships are shown to exist between the dose of rat serum used and the number of colonies produced and between the number of marrow cells plated and the number of colonies produced. Thus, the system is suitable for quantitative work with rat marrow cells. The nuclear morphology of cells in the colonies is described. The colonies initially consisted of cells with ring‐shaped nuclei but showed a transition to large mononuclear cells later in the incubation period.