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THE IMMUNE RESPONSES OF NORMAL AND TOLERANT RATS TO SALMONELLA ADELAIDE FLAGELLIN IN FREUND'S COMPLETE ADJUVANT
Author(s) -
Lind Patricia E
Publication year - 1968
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1968.15
Subject(s) - flagellin , adjuvant , antigen , antibody , immune system , salmonella , antibody response , immunology , freund's adjuvant , medicine , microbiology and biotechnology , biology , bacteria , receptor , genetics
Summary Antibody production in the rat in response to the injection of Salmonella adelaide flagellin was considerably enhanced if the antigen were Injected In emulsion with Freund's complete adjuvant. Peak titres of antibody Were 1000‐fold higher than those obtained following the injection of antigen alone. However the minimal dose of flagellin. which would stimulate antibody production was found to be of the order of 10 ng. (10 −8 g.) regardless of whether it were injected in adjuvant or in aqueous solution. Simultaneous injection of flagellin and adjuvant into opposite sides of the body resulted in only a very moderate enhancement of the antibody response. Rats which had been given a ten‐week course of bi‐weekly injections of flagellin starting in the neonatal period, and which were virtually unresponsive to subsequent challenge with flagellin, broke tolerance and produced high titres of antibody when challenged with the same dose of flagellin in adjuvant. These results have been interpreted in terms of the ability of antigen injected in Freund's complete adjuvant to be retained in the body for a considerable period of time where it remains available to stimulate both cells which “escape” the state of immune unresponsiveness and newly recruited antigen‐ sensitive cells whose proliferation is accelerated by the presence of the adjuvant.