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THE ZINC CONTENT OF RAT MAST CELLS
Author(s) -
Angyal Annette M,
Archer GT
Publication year - 1968
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1968.10
Subject(s) - zinc , mast cell , lysis , mast (botany) , peritoneal cavity , centrifugation , chemistry , biology , biochemistry , immunology , anatomy , organic chemistry
Summary The zinc content of isolated rat mast cells was estimated by atomic absorption spectrophotometry. The mast cells were harvested by peritoneal lavage and separated from other peritoneal cavity cells by density gradient centrifugation. The zinc content of large mast cells was found to be 2·1 μg. Zn/10 6 mast cells (standard error ± 0·23) and 40 times greater than the zinc content of other cell types. Small immature mast cells were found to contain less zinc than mature mast cells. Significant amounts of zinc were released from mast cells by freezing and thawing but not by hypotonic lysis.