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THE EFFECTS OF HOMOLOGOUS AND HETEROLOGOUS WHOLE SERUM UPON MULTIPLICATION OF RECENTLY‐ ISOLATED HUMAN SYNOVIAL CELLS IN CULTURE
Author(s) -
Clarris BJ,
Fraser JRE
Publication year - 1967
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1967.54
Subject(s) - heterologous , subculture (biology) , fetal bovine serum , homologous chromosome , biology , immunology , cell culture , microbiology and biotechnology , chemistry , andrology , biochemistry , medicine , genetics , gene
Summary The growth of recently established strains of human synovial cells has been studied in a system designed in measure rates of multiplication after cellular attachment has occurred during subculture. It was found that cell number declined in synthetic growth medium or balanced salt solution, and that the addition of heat‐inactivated serum arrested this decline or stimulated multiplication depending upon the concentration. Heterologous serum (bovine and fetal calf) was approximately as effective human serum for this purpose, in contrast with the superiority of the former in promotion of cellular attachment and spreading. Growth‐rates of synovial cells were relatively slow in concentrations of serum customary in cell culture. Higher concentrations were well tolerated, and greatly increased rates of multiplication.