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IMMUNOLOGICAL RESPONSES IN RATS FOLLOWING ANTIGENIC STIMULATION OF PEYER'S PATCHES
Author(s) -
Cooper GN,
Turner Kaye
Publication year - 1967
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1967.36
Subject(s) - antigen , lymphatic system , mesenteric lymph nodes , peyer's patch , biology , immunology , antibody , lymph node , microbiology and biotechnology , immune system
Summary A technique for inoculating small amounts of particulate antigens in the Peyer's of rats is described. When sheep red cells or their stroma an used, significant amount of the antigen are retained in the Peyer's patch and a draining mesenteric lymph node; while relatively small amounts of I 125 labelled flagella particles are retained in these tissues, very little of this antigen can be detected in other lymphoid tissues of the animals 48 hr. after injection. Serum antibody responses following injection of both antigens are characterized by a rapid development reaching maximal levels in 6–8 days and a relatively rapid decline over a period of 2–5 weeks in both instances a relationship between the antigen dose and the height and time of appearance of the maximal serum titres was observed. Using the Jerne technique for detection antibody‐producing cells, it seems that the response to sheep cell antigens (and presumably the flagella antigen) is confined to the injected Peyer's patch and its local draining mesenteric node. The potential value of this technique for studying certain aspects of the immunology reactivity of the gut‐associated lymphoid cells is discussed.