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THE EFFECT OF PENTOBARBITONE ANAESTHESIA ON INCREASED VASCULAR PERMEABILITY TO 131 I‐ALBUMIN IN GUINEA‐PIGS
Author(s) -
Garlick DG
Publication year - 1966
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1966.65
Subject(s) - histamine , albumin , anesthesia , factorial experiment , vascular permeability , evans blue , guinea pig , chemistry , permeability (electromagnetism) , medicine , endocrinology , biochemistry , mathematics , statistics , membrane
Summary The effect of sodium pentobarbitone anaesthesia on increased vascular permeability was investigated in guinea‐pigs. Increased permeability was indicated by the local exudation of circulating Evans blue dye and 131 I‐albumin in skin sites injected with histamine. Paired comparison tests and a factorial split‐plot design were used. Responses 10 minutes after histamine injections were measured as the amount of 131 I‐albumin per gram of skin per millilitre of blood. Anaesthetised animals were maintained in the first plane of surgical anaesthesia and warmed to maintain a constant body temperature. In unwarmed conscious animals the response was significantly less than that of the experimental (anaesthetised) group. The responses for the two groups were not significantly different when both anaesthetised and conscious animals were warmed. In a factorial experiment there was no significant interaction between the main effect of anaesthesia and the responses to three concentrations of histamine.