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NON‐SPECIFIC RESPONSES IN THE ASSAY OF THYROTROPHIN AND LONG‐ACTING THYROID STIMULATOR
Author(s) -
Adams DD,
Kennedy TH,
Purves HD
Publication year - 1966
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1966.34
Subject(s) - euthyroid , thyroid , endocrinology , medicine , antiserum , bioassay , globulin , hormone , albumin , antibody , immunology , biology , genetics
Summary In McKenzie's modification of Adams' and Purves' bioassay method for thyrotrophin and long‐acting thyroid stimulator serum samples from euthyroid people may elicit significant responses. These responses have a time course which differs from those of long‐acting thyroid stimulator and thyrotrophin. Unlike long‐acting thyroid stimulator, the activity is present in the albumin fraction of the serum proteins as well as in the gamma globulin fraction, and concentrates of the latter show no increase in activity over the serum. Other differences from human thyrotrophin are failure to be neutralized by rabbit antisera to human thyrotrophin and failure to disappear from people treated with thyroid hormone in suppressive dosage. It is concluded that the activity of euthyroid serum is not due to the presence of either long‐acting thyroid stimulator or thyrotrophin, and that the assay method is not specific for these substances.