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THE FORMATION OF MEMBRANES AROUND MICRO‐ORGANISMS AND PARTICLES INJECTED INTO AMOEBAE: SUPPORT FOR THE “RETICULOSOME” CONCEPT
Author(s) -
CasleySmith JR,
Savanat T
Publication year - 1966
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1966.13
Subject(s) - amoeba (genus) , membrane , vesicle , vacuole , cytoplasm , electron microscope , spore , biophysics , bacteria , chemistry , organelle , biology , microbiology and biotechnology , biochemistry , physics , genetics , optics
Summary Spores, bacteria, polystyrene and carbon particles were injected into Amoeba protons and the results followed with electron microscope. The particles initially lay free in the cytoplasm, but after two hours they became surrounded by a shell of granules morphologically resembling ribosomes, but they were not attacked by ribonnclease. These bodies appeared to be transformed gradually into membranes. It is considered that they may represent preformed portions of membrane, the reticulosomes of Pollak. If the amoebae were incubated at 4° in place of 20° the particles still surrounded the injected material, but membranes were not formed. The bacteria and the interiors of the spores were digested, and it was considered that numerous vesicles carrying moderately electron‐dense materials contributed to this digestion. Acid phosphatase was demonstrated in the vesicles and in the vacuoles.