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EFFECT OF OPSONINS ON PHAGOCYTOSIS AND KILLING OF SALMONELLAE BY AMOEBA PROTEUS , AND THE USE OF PHAGE P 22 TO ELIMINATE SURPLUS EXTRACELLULAR BACTERIA
Author(s) -
Savanat T,
Pavillard ERJ
Publication year - 1964
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1964.59
Subject(s) - phagocytosis , opsonin , microbiology and biotechnology , amoeba proteus , salmonella , antibody opsonization , amoeba (genus) , bacteria , extracellular , phagosome , biology , lytic cycle , intracellular , phagolysosome , intracellular parasite , proteus , virology , escherichia coli , biochemistry , genetics , virus , gene
Summary Mammalian serum opsonins have been shown to possess moderate power to promote phagocytosis of Salmonella enteritidis by Amoeba proteus , but serum opsonins had no measurable effect on the rate of intracellular killing of these bacteria or of Salmonella typhimurium. The survival time of opsonised salmonellae after phagocytosis by amoebae was considerably longer than would be expected if similar bacteria were to be ingested by mammalian phagocytes. The possible effect of temperature on intracellular bactericidal mechanisms is discussed. Also described is a technique in which a lytic mutant of phage P 22 was used to eliminate residual extracellular salmonellae from washed amoebae after a standard period of phagocytosis.