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THE ABILITY OF AMOEBA PROTEUS TO KILL SALMONELLA ENTERITIDIS INTRODUCED BY MICRO‐INJECTION, AND THE INFLUENCE OF OPSONINS ON INTRACELLULAR KILLING
Author(s) -
Savanat T,
Pavillard ERJ
Publication year - 1964
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1964.58
Subject(s) - amoeba proteus , salmonella enteritidis , amoeba (genus) , microbiology and biotechnology , opsonin , proteus , salmonella , intracellular , biology , bacteria , phagocytosis , escherichia coli , biochemistry , genetics , gene
Summary A micro‐injection technique is described which allowed S. enteritidis Se795 to be injected directly into the cytoplasm of Amoeba proteus . The decrease in the number of bacteria recovered after increasing intervals of time allowed that the half‐life of S. enteridis within amoebae was approximately five hours. Opsonisation of the salmonellae with serum did not shorten their intracellular half‐life.