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INSULIN AND MYELOSCHISIS IN THE CHICK EMBRYO
Author(s) -
Dongen Robert
Publication year - 1964
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1964.57
Subject(s) - neural tube , medicine , embryo , anatomy , biology , genetics
Summary Injections of various insulin doses were made in two series of eggs—at the 24‐ and 48‐hour stage of development respectively. The insulin was introduced directly into or very near to the subgenninal cavity. In the 24‐hour series, the main abnormalities encountered were rumplessness, microphthalmia and myeloschisis. All but myeloschisis occur just as frequently in control eggs injected with distilled water. Raising the dose from 1/100 to 1/20 units had no obvious effect on the incidence of abnormalities. Doses of 1/10 units are on the borderline between teratogenesis and death. At 48 hours, only a low incidence of myeloschisis was found. This is probably associated with the fact that closure of the neural tube is nearing completion at this time. The aetiology of the increased neural bulk observed in myeloschisis is at present unknown.