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STOP‐FLOW ANALYSIS OF PHENOLSULPHONPHTHALEIN EXCRETION AND VISUAL LOCALIZATION OF THE SITE OF MAXIMAL URINE ACIDIFICATION IN THE RAT NEPHRON
Author(s) -
Young JA,
Edwards KDG
Publication year - 1964
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1964.50
Subject(s) - nephron , reabsorption , diuresis , chemistry , urine , tubule , renal function , convoluted tubule , excretion , renal sodium reabsorption , kidney , sodium , endocrinology , creatinine , medicine , biology , biochemistry , organic chemistry
Summary Stop‐flow analysis of renal tubular function performed in the rat undergoing creatinine diuresis has been used to demonstrate active secretion of phenolsulphonphthalein by the proximal convoluted tubule. At the same time, by taking advantage of this indicator's colour change from yellow to red with rising pH, it has proved possible to localize the region of maximal tubular acidification of urine by simple inspection of the colour of the stop‐flow samples. This region was found to coincide with the region of maximum sodium and water reabsorption, and was thought to be in the collecting ducts.