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AUTORADIOGRAPHIC STUDIES OF NUCLEIC ACID AND PROTEIN METABOLISM IN LYMPHOID CELLS
Author(s) -
Mitchell Judith
Publication year - 1964
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1964.35
Subject(s) - rna , nucleic acid , protein biosynthesis , in vitro , uridine , lymph node , microbiology and biotechnology , leucine , in vivo , dna synthesis , dna , biochemistry , biology , cytoplasm , cell , chemistry , amino acid , immunology , genetics , gene
Summary Short pulse labelling of lymph node cells with 3 H‐uridine in vitro showed that blast cells retained the label they had acquired for at least a further six hours in non‐radioactive medium. However, within the cell, a decrease in labelled nuclear RNA was observed, with a corresponding increase in labelled cytoplasmic RNA and nuclear DNA. 3 H‐leucine pulse experiments showed that the mature plasma cells secrete labelled protein faster than blast cells, whilst small lymphocytes appear to make only small amounts of fairly stable protein. The differences between the behaviour of the lymph node cells in protein synthesis in vitro were confirmed in vivo . Studies with actinomycin D showed that all RNA synthesis in blast cells could be uncoupled from 70 per cent of the protein synthesis during three hours′ exposure to the drug. Of the blast cell's RNA, 25 per cent seemed to be more sensitive than the rest to low concentrations of the drug.