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THE EXPRESSION OF HETEROPHILE ANTIGENICITY BY SALMONELLAE
Author(s) -
Collins FM,
Rowley Derrick
Publication year - 1964
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1964.26
Subject(s) - subculture (biology) , fowl , microbiology and biotechnology , biology , horse , hemagglutination , antiserum , antigenicity , antibody , virology , immunology , paleontology
Summary S. enteritidis and S. pullorum‐gallinarum were grown in mouse or fowl liver homogenate in 30 per cent serum broth for 10 successive subcultures. Fowls were immunised with suspensions of these organisms after one subculture in a defined casamino acid medium. Increased titres of haemagglutinating antibodies against mouse and rabbit red cells were detected in the fowl antisera. Cultures of both organisms grown in 30 per cent horse, pig, shark, kangaroo, rabbit or human serum broth all strongly absorbed the fowl serum haemagglutinins. The original strain of S. enteritidis could absorb the fowl serum haemagglutinins after one subculture in 30 per cent horse serum broth. This absorptive ability decreased sharply after one subculture in the synthetic medium.