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FACTORS INVOLVED IN THE ADHERENCE OF S. TYPHIMURIUM C5 AND MOUSE PERITONEAL MACROPHAGES
Author(s) -
Auzins Ieva,
Rowley D
Publication year - 1963
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1963.44
Subject(s) - bacteria , microbiology and biotechnology , macrophage , antigen , organism , suspension (topology) , biology , phagocytosis , chemistry , immunology , in vitro , biochemistry , paleontology , genetics , mathematics , homotopy , pure mathematics
SUMMARY A technique has been evolved whereby the rate of attachment or adherence of bacteria to mouse peritoneal macrophages can be studied. The aim of the technique was to obtain samples, taken at intervals after mixing a suspension of bacteria and macrophages, which contained bacteria not yet associated with cells. This was achieved by passing the test suspensions through filters holding back macrophages but allowing tin‐ free passage of bacteria not attached to these cells. Using this method no association of S. typhimurium C5 with macrophages occurs unless the organisms have been opsonised. It has also been shown that there are one or more heat‐labile antigens present on the surface of this organism. These must be covered before the organism will adhere to the surface of mouse macrophages.