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ACTIONS OF METAL CHELATES OF SUBSTITUTED 1,10‐PHENANTHROLINES ON VIRUSES AND CELLS
Author(s) -
White David O,
Harris Alan W,
Shulman Albert
Publication year - 1963
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1963.43
Subject(s) - chelation , virus , chemistry , acetylacetone , in vitro , hela , metal , virology , biology , biochemistry , organic chemistry
SUMMARY An assay system has been developed for the rapid and accurate titration of the virus‐inhibitory activity of antiviral agents. Metal chelates of substituted 1,10‐phenanthrolines, at concentrations well below those lethal to virus, inhibit the growth of influenza virus in chick embryo allantois‐on‐shell in vitro by acting on the host cell. Amongst a Large group of metal chelates there are significant relationships between chemical structure and virus‐inhibitory activity. The most active compound, [Ru(3,5,6,8‐tetramethylphen) 2 acetylacetone] + , at a con centration as low as 10 −6 M, completely and irreversibly inhibits the growth of up to 10,000 ID 50 of virus within 20 minutes of contact with cells. It has no effect on adsorption or penetration of virus into the cell, but acts during the eclipse period to prevent the synthesis of new virus. All concentrations of [Ru(3,5,6,8‐tetramethylphen 2 acetylacetone] + inhibiting viral repro duction also depress the utilization of oxygen by the cells.