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STUDIES ON THE VIRUS OF RABBIT PAPILLOMA
Author(s) -
White David O,
Huebner Robert J,
Rowe Wallace P,
Traub Renée
Publication year - 1963
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1963.3
Subject(s) - complement fixation test , infectivity , antibody , virology , virus , antigen , biology , titration , chemistry , immunology , serology , inorganic chemistry
SUMMARY Methods for the accurate assay of rabbit papilloma virus and its antibody are described. Viral infectivity is titrated by a technique in which up to fifty discrete tumours may be counted on a single inoculation site. Neutralizing antibody is measured with considerable accuracy by determining the dilution of serum producing a 50 p.c. reduction in tumour count. A complement fixation test designed to economize on reagents permits the quick and accurate titration of RPV antigen and antibody. Some of the relationships between tumours, infectious virus, antigen and antibody in naturally and experimentally infected rabbits are examined.