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STUDIES OF CYTOTOXICITY USING P 32
Author(s) -
Forbes IJ
Publication year - 1963
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1963.25
Subject(s) - cytotoxic t cell , cytotoxicity , cell culture , microbiology and biotechnology , antiserum , chemistry , fibroblast , cell , kidney , in vitro , biology , pathology , immunology , biochemistry , endocrinology , medicine , antibody , genetics
SUMMARY A technique is described for detecting damage to cells in culture using P 32 . The method is relatively simple and results obtained with it are reproducible. The method depends on the principle that P 32 passes from cells into a non‐toxic medium at a rate which is a property of the particular type of cell. Cytotoxic agents cause an increased rate of leakage of P 32 from cells. The method was satisfactory with all of the cultures which were studied: the L‐fibroblast, primary cultures of the 16–6 rat thyroid carcinoma and rat kidney, and subcultures of these rat cells. Four types of cytotoxic agents were shown to cause an increased loss of P 32 from the cells on which they were tested: the natural rat cytotoxin in rabbit serum, NaOH, antisera to rat tissues, and mechlorethamine hydrochloride (“Mustargen”). Data are presented to show that increased P 32 loss is due to cell damage.