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THE ESTIMATION OF TESTOSTERONE IN BIOLOGICAL FLUIDS
Author(s) -
Hudson Bryan,
Coghlan John,
Dulmanis Ausma,
Wintour Marelyn,
Ekkel Ida
Publication year - 1963
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1963.23
Subject(s) - testosterone (patch) , isotope dilution , steroid , acetic anhydride , derivative (finance) , chromatography , chemistry , hormone , endocrinology , medicine , biology , biochemistry , mass spectrometry , financial economics , economics , catalysis
SUMMARY A method is described for the estimation of free testosterone in human peripheral plasma. This method which uses the double isotope dilution derivative principle with C 14 ‐testosterone as marker steroid, H 3 ‐acetic anhydride as deriving agent, shows normal male levels to range from 0.49–0.98 μg./100 ml. and females from less than 0.05 μg./1OO ml. to 0.31 μg./100 ml. The results obtained by this method have been compared with those given by other techniques for the estimation of this steroid hormone.