z-logo
Premium
THE ESTIMATION OF TESTOSTERONE IN BIOLOGICAL FLUIDS
Author(s) -
Hudson Bryan,
Coghlan John,
Dulmanis Ausma,
Wintour Marelyn,
Ekkel Ida
Publication year - 1963
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1963.23
Subject(s) - testosterone (patch) , isotope dilution , steroid , acetic anhydride , derivative (finance) , chromatography , chemistry , hormone , endocrinology , medicine , biology , biochemistry , mass spectrometry , financial economics , economics , catalysis
SUMMARY A method is described for the estimation of free testosterone in human peripheral plasma. This method which uses the double isotope dilution derivative principle with C 14 ‐testosterone as marker steroid, H 3 ‐acetic anhydride as deriving agent, shows normal male levels to range from 0.49–0.98 μg./100 ml. and females from less than 0.05 μg./1OO ml. to 0.31 μg./100 ml. The results obtained by this method have been compared with those given by other techniques for the estimation of this steroid hormone.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here