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THE MICRO‐DETERMINATION OF BLOOD GLUCOSE
Author(s) -
Watson D,
Stevenson MEK
Publication year - 1963
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1963.21
Subject(s) - glucose oxidase , blood sugar , preservative , chromatography , chemistry , whole blood , pharmacology , diabetes mellitus , biochemistry , medicine , food science , endocrinology , enzyme
SUMMARY A detailed examination of four modern manual methods for determining blood glucose is presented, and the effects of preservatives, anticoagulants, drugs and some reducing or interfering substances on the estimations are indicated. On grounds of precision, reliability and simplicity, the o‐amino‐toluene condensation method of Hultman and the glucose oxidase‐tolidine method of Watson are the most satisfactory and the Cu‐reduction method of Nelson the least satisfactory means of determining blood sugar in the clinical laboratory. Blood cells from normal and diabetic patients contain 65 p.c. (± 10 p.c.) of the glucose concentration found in the blood plasma of the same subject.