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THE FORMATION OF PLAQUES IN TISSUE CULTURE BY ARBOVIRUSES
Author(s) -
Miles JAR,
Austin FJ
Publication year - 1963
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1963.19
Subject(s) - arbovirus , tissue culture , titer , agar , protamine , biology , virology , dextran , microbiology and biotechnology , chemistry , virus , bacteria , biochemistry , genetics , in vitro , heparin
SUMMARY A number of arboviruses multiply in avian cells in tissue culture but fail to produce plaques in them when grown under an agar overlay. It has been shown that the addition of optimal amounts of DEAE dextran or of protamine enables some such viruses to form clear‐cut plaques to a high titre. The optimal doses do not inhibit plaque production by any arbovirus tested.

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