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EFFECTS OF SIMPLE LIPIDS ON THE PHAGOCYTIC PROPERTIES OF PERITONEAL MACROPHAGES
Author(s) -
Cooper GN,
West Dawn
Publication year - 1962
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1962.52
Subject(s) - triolein , antibody opsonization , stimulation , incubation , in vitro , macrophage , chemistry , peritoneal cavity , incubation period , immunology , endocrinology , biology , biochemistry , anatomy , enzyme , lipase , opsonin
SUMMARY Using an in vitro phagocytic system, stimulation of macrophage activity following exposure of mouse peritoneal cells to glyceryl trioleate (triolein) suspension has been found. The stimulation is time‐ and dose‐dependent, maximum effects on 2 × 10 6 cells being obtained with 50 μg. triolein for 60 minutes. Larger doses of triolein are not immediately stimulatory but following prolonged incubation the cells eventually develop significant activity. The stimulation is due to contact between the cells and triolein particles and is independent of the degree of opsonization of the organisms by normal animal sera.

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