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A COMPARISON OF THE METABOLISM OF PALMITIC, OLEIC AND LINOLEIC ACIDS INCORPORATED INTO CHYLOMICRON TRIGLYCERIDES
Author(s) -
SimpsonMorgan MW,
Morris Bede
Publication year - 1962
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1962.49
Subject(s) - chylomicron , palmitic acid , oleic acid , chemistry , linoleic acid , metabolism , excretion , biochemistry , triglyceride , chromatography , food science , fatty acid , cholesterol , very low density lipoprotein , lipoprotein
SUMMARY The metabolism of chylomicron triglycerides containing predominantly palmitic, oleic and linoleic acids has been studied in intact rats and in isolated perfused rats’ livers using 14 C‐labelled chylomicrons. In intact rats infused continuously with labelled chylomicrons the rate of excretion of 14 CO 2 reached a relative plateau during the second hour of the infusion. The rate of excretion of 14 CO 2 in the third and fourth hours of the infusion showed that about 40 p.c. of the labelled fat was converted to CO 2 as it was infused. It was found that significantly less palmitic acid was oxidized to CO 2 than oleic or linoleic acids. This difference was, however, small. About 4 p.c. of the radioactivity of the infused labelled fat underwent recycling and subsequent oxidation once the relative plateau was reached. In experiments with perfused livers there was no significant difference in the rate of uptake or oxidation of palmitic, oleic or linoleic acids incorporated into chylomicrons. Significantly more linoleic acid than oleic or palmitic acid was incorporated into the phospholipids of the perfusate. The results showed that the rate of oxidation of palmitic, oleic or linoleic acids incorporated into chylomicrons can be used as a measure of the overall rate of oxidation of chylomicron triglycerides.