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THE MICROBIOLOGICAL ASSAY OF SERUM FOLIC ACID ACTIVITY
Author(s) -
Davis RE,
Kelly A
Publication year - 1962
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1962.48
Subject(s) - lactobacillus casei , folic acid , ascorbic acid , chemistry , blood serum , biochemistry , medicine , food science , fermentation
SUMMARY A microbiological assay for serum folic acid activity using Lactobacillus casei as the test organism is described. The method uses ascorbic acid to protect the folic acid‐like activity of serum and is sensitive enough to differentiate between sera from normal and folic acid‐deficient subjects without overlap. One hundred normal sera have been assayed and fall within the range 2·7–18·5 mμg./ml. There does not appear to be a significant difference between male and female serum levels.