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THE EFFECT OF ANTIOXIDANTS ON POISONING BY CARBON TETRACHLORIDE
Author(s) -
Gallagher CH
Publication year - 1962
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1962.28
Subject(s) - carbon tetrachloride , chemistry , selenium , vitamin e , antioxidant , pyridine , necrosis , stomach tube , pharmacology , tocopherol , sodium selenate , sodium , biochemistry , medicine , medicinal chemistry , organic chemistry , anatomy
SUMMARY The prior administration of the antioxidants α‐tocopherol acetate, sodium selenite or NN 1 ‐diphenyl‐p‐phenylenediamine (DPPD) afforded protection to rats given lethal doses of carbon tetrachloride. Under the experimental conditions DPPD appeared to be the most effective of the three antioxidants tested. A single dose of 100 mg. DPPD injected intraperitoneally at the same time as CCl 4 was administered by stomach tube proved more effective than one dose of 95 international units of vitamin E given simultaneously with CCl 4 , or than a dose of 95 I.U. given simultaneously and another five hours later. Sodium selenite was less effective than the other two antioxidants. When antioxidants were given, pyridine nucleotide concentrations in the liver were maintained despite the subsequent administration of CCl 4 ., but in their absence concentrations of both oxidized and reduced pyridine nucleotides in the liver fell by more than 50 p.c. The loss of reduced pyridine nucleotides was completely prevented by DPPD, the only antioxidant studied in this regard, and the loss of oxidized pyridine nueleotides was largely prevented by DPPD or α‐tocopherol acetate and to a lesser degree by sodium selenite. Three doses of 100 mg. DPPD at 48, 24 and 0 hours before rats were given 4 ml. CCl 4 /kg. prevented necrosis of liver cells and diminished the fatty change appreciably. A single dose of 100 mg. DPPD injected intraperitoneally 48 hours before 4 ml. CCl 4 /kg. also largely prevented hepatic necrosis and decreased the fatty change.