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GLUCOSE METABOLISM IN HUMAN (THIERSCH) SKIN GRAFTS
Author(s) -
Opit LJ,
Savage JP
Publication year - 1961
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1961.59
Subject(s) - glycogen , glycolysis , metabolism , carbohydrate metabolism , oxygen , in vivo , human skin , respiration , in vitro , dinitrophenol , chemistry , biochemistry , glucose uptake , substrate (aquarium) , incubation , biology , endocrinology , anatomy , ecology , genetics , microbiology and biotechnology , organic chemistry , insulin
SUMMARY Glucose metabolism in human split skin (Thiersch) grafts, when measured in vitro , is shown to be mainly by glycolysis with a low rate of oxygen uptake. Doubling of glucose uptake and lactate production as well as oxygen uptake by 0·1 mM 2:4‐dinitrophenol (DNP) is interpreted as supporting the conception that this glycolytic activity is not simply due to inadequate oxygen perfusion but is a feature of skin metabolism in vivo . The ability of skin to maintain an oxygen uptake for at least four hours without substrate suggested that skin glycogen might be the endogenous carbohydrate capable of sustaining respiration but measurements of glycogen levels in skin before and after incubation demonstrate that glycogen itself is unlikely to provide this pool, at least in the in vitro system.